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Nupore Selection Chart For Blotting Membranes
This selection chart highlights the suitability of various Nupore blotting membranes for different applications based on their properties.
| Membrane Type | SCN Pure Cellulose Nitrate |
SNN Pure Nylon-66 |
|---|---|---|
| Biomolecules | ||
| Nucleic Acids | R | HR |
| Proteins | R | R |
Transfer Method |
||
| Dot Blot | R | R |
| Colony or Plaque Lift | HR | R |
| Electrotransfer | R* | HR |
| Capillary Blot | R | R |
| Vaccum Blot | R | R |
| Alkaline Transfer | NR | R |
Molecule Fixation |
||
| Baking | R | R |
| Drying | R | R |
| UV Crosslinking | P | HR |
| Alkali Fixation | NR | R |
| Molecule Removal | NR | NR |
Detection Method |
||
| Colorimetric | HR | R |
| Radiolabelled | R | R |
| Luminescent | R | P |
| Fluorescent | R | P |
| Staining | R | P |
Reprobing |
||
| Once | NR | R |
| Multiple | NR | R |
HR = Highly Recommended
R = Recommended
R* = Recommended for Proteins only
P = Possible
NR = Not Recommended